bio

Constantinos Broustas, Ph.D.

Assistant Professor of Radiation Oncology

Center for Radiological Research

Contact Information: 

Center for Radiological Research
630 West 168th Street
New York, NY 10032

Telephone: (212) 305-2187

Email: cgb2117@cumc.columbia.edu

 

Education

BS, Chemistry, National and Capodistrian University of Athens.

MS, Biological Chemistry, University of Michigan, Ann Arbor.

PhD, Biological Chemistry, University of Michigan, Ann Arbor.

Postdoctoral Fellow, Cancer Biology, Georgetown University, Washington, DC.

 

Research

Cross-talk between growth factor signaling and DNA damage response in cancer and therapeutic resistance. Research in my laboratory focuses on the elucidation of the mechanisms by which pro-survival signaling pathways, such as MAP kinases and PI3K/Akt, affect the DNA damage response and repair machinery to regulate processes critical for maintaining genome integrity, as well as confer tumor resistance to genotoxic stress. Specifically, we seek to determine the contribution of mitogen/extracellular signal-regulated kinase kinase-5 (MEK5), a member of the MAP kinase family, in the induction of radioresistance in human prostate tumors and perform mechanistic studies, using cancer cell lines and animal models. Ultimately, we aim at developing a small molecule inhibitor of MEK5 that in combination with radiation will radiosensitize tumor cells and maximize the curative potential of radiation therapy for patients with localized prostate cancer.

The impact of aging on radiation biodosimetry. The goal of radiation biodosimetry is to accurately predict radiation dose and type, as a surrogate of radiological injury, in a large-scale radiation emergency. Gene expression profiling represents a promising method of biodosimetry. However, for a gene expression signature to be useful, it should be applicable across diverse populations irrespective of age, gender, or environmental and genetic backgrounds. Aging is characterized by random and widespread unrepaired damage to DNA, as well as gene expression changes that may compromise the predictive value of radiation biodosimetry. Our studies are designed to investigate the impact of aging on the response to radiation, by using total body irradiation combined with transcriptomics analysis of whole blood cells from young and old mice, as well as ex vivo irradiated human blood. The results from this study will help define a consensus gene signature that is valid across various age groups.

 

Selected Activities

Member of Radiation Research Society, 2011-present.

 

Publications

Broustas CG, Xu Y, Harken AD, Chowdhury M, Garty G, Amundson SA. Impact of Neutron Exposure on Global Gene Expression in a Human Peripheral Blood Model. Radiat Res. 2017;187(4):433-440.

Lieberman HB, Panigrahi SK, Hopkins KM, Wang L, Broustas CG. p53 and RAD9, the DNA Damage Response and Regulation of Transcription Networks. Radiat Res. 2017;187(4):424-432.

Broustas CG, Xu Y, Harken AD, Garty G, Amundson SA. Comparison of gene expression response to neutron and x-ray irradiation using mouse blood. BMC Genomics. 2017;18(1):2.

Broustas CG, Lieberman HB. RAD9 enhances radioresistance of human prostate cancer cells through regulation of ITGB1 protein levels. Prostate. 2014;74(14):1359-70.

Broustas CG, Lieberman HB. DNA damage response genes and the development of cancer metastasis. Radiat Res. 2014;181(2):111-30.

Broustas CG, Zhu A, Lieberman HB. Rad9 protein contributes to prostate tumor progression by promoting cell migration and anoikis resistance. J Biol Chem. 2012;287(49):41324-33.

Broustas CG, Lieberman HB. Contributions of Rad9 to tumorigenesis. J Cell Biochem. 2012;113(3):742-51.

Lieberman HB, Bernstock JD, Broustas CG, Hopkins KM, Leloup C, Zhu A. The role of RAD9 in tumorigenesis. J Mol Cell Biol. 2011;3(1):39-43.

Broustas CG, Ross JS, Yang Q, Sheehan CE, Riggins R, Noone AM, Haddad BR, Seillier-Moiseiwitsch F, Kallakury BV, Haffty BG, Clarke R, Kasid UN. The proapoptotic molecule BLID interacts with Bcl-XL and its downregulation in breast cancer correlates with poor disease-free and overall survival. Clin Cancer Res. 2010;16(11):2939-48.

Cavalli LR, Santos SC, Broustas CG, Rone JD, Kasid UN, Haddad BR. Assignment of the BLID gene to 11q24.1 by fluorescence in situ hybridization. Cancer Genet Cytogenet. 2008;186(2):120-1.

Boudreau HE, Broustas CG, Gokhale PC, Kumar D, Mewani RR, Rone JD, Haddad BR, Kasid U. Expression of BRCC3, a novel cell cycle regulated molecule, is associated with increased phospho-ERK and cell proliferation. Int J Mol Med. 2007;19(1):29-39.

Broustas CG, Gokhale PC, Rahman A, Dritschilo A, Ahmad I, Kasid U. BRCC2, a novel BH3-like domain-containing protein, induces apoptosis in a caspase-dependent manner. J Biol Chem. 2004;279(25):26780-8.

Kumar D, Gokhale P, Broustas C, Chakravarty D, Ahmad I, Kasid U. Expression of SCC-S2, an antiapoptotic molecule, correlates with enhanced proliferation and tumorigenicity of MDA MB 435 cells. Oncogene. 2004;23(2):612-6.

Broustas CG, Grammatikakis N, Eto M, Dent P, Brautigan DL, Kasid U. Phosphorylation of the myosin-binding subunit of myosin phosphatase by Raf-1 and inhibition of phosphatase activity. J Biol Chem. 2002;277(4):3053-9.

Broustas CG, Larkins LK, Uhler MD, Hajra AK. Molecular cloning and expression of cDNA encoding rat brain cytosolic acyl-coenzyme A thioester hydrolase. J Biol Chem. 1996;271(18):10470-6.

Broustas CG, Hajra AK. Purification, properties, and specificity of rat brain cytosolic fatty acyl coenzyme A hydrolase. J Neurochem. 1995;64(5):2345-53.

 

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